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Figure 6 | BMC Biotechnology

Figure 6

From: Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres

Figure 6

Gene expression profile of three different stem cell lines after five passages in USSC growth mediumACF. RT-PCR for pluripotency (Sox2), ectodermal (Pax6), mesodermal (Gata6) and endodermal (Brachyury), associated genes after five passages in USSC growth mediumACF. The house keeping gene GAPDH was used as a positive control. Lane 1, hES cells; Lane 2, RT-negative control for hES cells. Lanes 3-8 are USSCs cultured in fetal calf serum: Lane 3, USSC Line 1; Lane 4, RT-negative control for USSC Line 1; Lane 5, USSC Line 2; Lane 6, RT-negative control for USSC Line 2; Lane 7, USSC Line 3; and Lane 8, RT-negative control for USSC Line 3. Lanes 9-14 are USSCs passaged five times in USSC growth mediumACF: Lane 9, USSC Line 1; Lane 10, RT-negative control for USSC Line 1; Lane 11, USSC Line 2; Lane 12, RT-negative control for USSC Line 2; Lane 13, USSC Line 3; and Lane 14, RT-negative control for USSC Line 3.

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