Table 1 Single-copy integration rate of tagged high-expressing cells
From: Recombinant protein expression by targeting pre-selected chromosomal loci
Retroviral | Plasmidal (transfer method) | |||
|---|---|---|---|---|
HEK293 | 44/48 | 92% | 5/9 (EP Gene Pulser, BioRad) | 56% |
CHO-K1 | 2/2 | 100% | 6/20 (Nucleofection, Amaxa) | 30% |