Figure 3
From: Recombinant protein expression by targeting pre-selected chromosomal loci
Gene expression of cell clones after targeting by cassette exchange. Characterization of HP-GFP and CP-GFP upon targeting. The cell clones show homogeneous loss of GFP expression after targeting with an antibody expression vector as depicted in (B). (B) Molecular characterization of the targeting event by PCR. Amplification of a 550 bp fragment from the daughter cell clones (D1-D6) using primers located at the indicated positions (arrows) is specific for integration of the targeting construct (P: parental tagged cell clone). (C) Homogeneity of the targeted daughter clones. The expression level of daughter clones after cassette exchange is compared with the expression level of the parental clones. Upper left: CP-HTG tagged clone targeted with a SV40 promoter driven GFP cassette. Upper right: HRV-GFP tagged clone targeted with a CMV-RFP cassette. Antibody targeting: CP-HTG tagged clone (lower left) and CP-AbHTG tagged clone (lower right) were targeted with an antibody expression construct as depicted in (B). The expression of the targeted daughter clones is shown.