Skip to main content
Figure 2 | BMC Biotechnology

Figure 2

From: Recombinant protein expression by targeting pre-selected chromosomal loci

Figure 2

Characterization of the tagged integration sites. (A) GFP expression pattern of single copy HEK293 and CHOK1 clones obtained upon retroviral and plasmidic transfer of the tagging cassette. Representative clones are shown. Green lines: tagged clones. Grey lines represent non-transfected HEK293 and CHOK1 cells, respectively. (B) CHO cells were tagged with an antibody expression construct. The antibody expression level production was evaluated. Only cell clones that produced >2 pcd are shown; the data were obtained from 3-5 replicates. Clones showing single copy integration events (data not shown) are marked by asterisks. Their GFP expression profile is given presented on the right. Green lines: tagged clones; grey lines: non-transfected CHOK1 cells. Nomenclature: HRV-GFP: HEK293 cells retrovirally tagged with eGFP (RV-GFP according to figure 1B); HP-GFP: HEK293 cells plasmid-tagged with eGFP (P-GFP); CRV-GFP: CHO cells retrovirally tagged with eGFP (RV-GFP); CP-AbHTG: CHO cells plasmid- tagged with antibody cassette (PAb-HTG).

Back to article page