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Table 5 Comparison of lentiviral vector titers using different cell lines

From: Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography

Cell line

HOS

293T

HeLa

Titer

3.1 ± 0.3 × 1010 TU/ml

2.0 ± 0.3 × 1010 TU/ml

9.0 ± 3.4 × 109 TU/ml

  1. Freshly concentrated LV vector stocks were diluted and aliquots were used to transduce HOS, 293T and HeLa cells as outlined under Materials and Methods. Titers were determined by FACS (n = 3). Data are expressed as mean ± SD.
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BMC Biotechnology

ISSN: 1472-6750

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