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Table 2 Capacity of Mustang Q Acrodiscs for crude lentiviral vector stocks

From: Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography

Production vessels Input (TU) Unbound (TU) Bound (TU) Recovered (TU) Yield Capacity of Acrodisc (TU/ml)
150-cm2 dishes 1.2 ± 0.5 × 109 6.4 ± 5.1 × 108 5.5 ± 1.6 × 108 3.2 ± 0.8 × 108 28.9 ± 5.4% 3.0 ± 0.9 × 109
HYPERFlask 1.6 ± 0.1 × 1010 2.8 ± 6.4 × 108 1.6 ± 1.6 × 1010 1.2 ± 0.1 × 1010 76.0 ± 0.70% ≥8.7 ± 0.9 × 1010
  1. Mustang Q Acrodiscs were challenged with 75-ml aliquots of vector-containing supernatants. The crude vector supernatants were filtered using a 0.45 μm filter prior to anion exchange chromatography on Mustang Q Acrodiscs. Input corresponds to the total number of TU loaded. Unbound corresponds the total number of TU found in the flow-through. Bound represents input TU minus unbound TU. Recovered corresponds to the total number of TU following elution. Capacity corresponds to the total number of TU bound per ml of the Mustang Q membrane. Since the HYPERFlask-derived samples did not result in greater than 10% breakthrough, the upper limit of the capacity for these samples is not known. Titers were determined by FACS using HOS cells. Data from three independent experiments involving 150-cm2 dish-derived and HYPERFlask vessel-derived vector supernatants are shown. The data are expressed as mean ± SD.