Figure 4
Induction of BMSC-specific CD8+ T-cell responses following intramuscular, but not intracerebral, cell implantation in syngeneic immunocompetent mice. (A) Spleen CD8+ T-cells (1 × 105 cells/well) from non-transplanted ROSA26-L-S-L-Luciferase mice (control mice, n = 4) and ROSA26-L-S-L-Luciferase mice with BMSC-Luc implants, either intramuscularly (intramuscular BMSC-Luc graft, n = 5) or intracerebrally (intracerebral BMSC-Luc graft, n = 6), were cultured in quadruplet in an IFN-γ ELISPOT assay alone (unstimulated), with addition of parental BMSC (BMSC re-stimulation) or with addition of BMSC-Luc (BMSC-Luc re-stimulation) (1 × 104 cells/well, ratio 10:1). Data are expressed as the mean number of IFN-γ spot forming cells (SFC)/1 × 105 CD8+ T-cells for each experimental group. (B) Spleen CD8+ T-cells (1 × 105 cells/well) from non-transplanted ROSA26-L-S-L-Luciferase mice (control mice, n = 4) and ROSA26-L-S-L-Luciferase mice with BMSC-Luc/eGFP/Pac implants, either intramuscularly (intramuscular BMSC-Luc/eGFP/Pac graft, n = 5) or intracerebrally (intracerebral BMSC-Luc/eGFP/Pac graft, n = 6), were cultured in quadruplet in an IFN-γ ELISPOT assay alone (unstimulated), with addition of parental BMSC (BMSC re-stimulation) or with addition of BMSC-Luc/eGFP/Pac (BMSC-Luc/eGFP/Pac re-stimulation) (1 × 104 cells/well, ratio 10:1). Data are expressed as the mean number of IFN-γ spot forming cells (SFC)/1 × 105 CD8+ T-cells for each experimental group.