Figure 2

Survival of luciferase-expressing BMSC derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice. (A) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA26-L-S-L-Luciferase transgenic mice following implantation in the central nervous system of syngeneic immunocompetent mice. (B) Representative histological analysis of clonal BMSC-Luc grafts in the central nervous system of syngeneic immunocompetent mice. Week 1 post-implantation: Upper pictures, haematoxylin-eosin (HE) staining indicating localisation and general appearance of the implantation site. Lower left picture, Sca-1 staining indicating the BMSC origin of the observed cell graft. Lower right picture, CD11b staining indicating the presence of activated microglia surrounding the observed cell graft. Week 3 post-implantation: Upper pictures, HE staining indicating localisation and general appearance of the implantation site. Lower left picture, Sca-1 staining indicating the BMSC origin of the observed cell graft. Lower right picture, CD11b staining indicating the absence of activated microglia surrounding the observed cell graft. All slides were examined using a conventional bright field microscope and digital pictures were taken under magnification as indicated by the scale bars. (C) Representative time course for in vivo bioluminescence imaging of clonal BMSC-Luc derived from ROSA26-L-S-L-Luciferase transgenic mice following intramuscular implantation in syngeneic immunocompetent mice.