Table 6 Effect of different combinations of parameters on lipase production
No. | Temperature (°C) | Volume (ml) | Inoculum Size (%) | Agitation (rpm) | Time (h) | pH | Activity (U ml-1) |
|---|---|---|---|---|---|---|---|
1 | 63 | 200 | 4.6 | 60 | 43.2 | 5.4 | 0.15 |
2 | 47 | 200 | 2.2 | 20 | 24 | 7.4 | 0.15 |
2 | 53 | 200 | 4.2 | 80 | 57.6 | 7 | 0.15 |
3 | 59 | 65 | 3.8 | 100 | 24 | 7 | 0.15 |
5 | 45 | 50 | 1.8 | 160 | 48 | 6.2 | 0.2 |
6 | 47 | 80 | 1.8 | 180 | 28.8 | 6.6 | 0.2 |
7 | 61 | 50 | 2.6 | 60 | 33.6 | 5 | 0.2 |
8 | 57 | 125 | 3.4 | 0 | 72 | 5.8 | 0.2 |
9 | 47 | 95 | 1 | 20 | 38.4 | 6.6 | 0.3 |
10 | 49 | 50 | 4.6 | 0 | 38.4 | 5.4 | 0.3 |
11 | 53 | 80 | 2.2 | 100 | 24 | 6.2 | 0.3 |
12 | 55 | 50 | 3.4 | 60 | 28.8 | 6.6 | 0.3 |