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Table 5 Solution of optimum condition

From: A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM

No.

Growth temperature (°C)

Medium volume (ml)

Inoculum size (%)

Agitation rate (rpm)

Incubation period (h)

Initial pH

ANN predicted activity (Uml-1)

Actual activity ± SD (Uml-1)

RSM predicted activity (Uml-1)

1

52.3

50

1

0

24

5.8

0.47

0.47 ± 0.003

0.476

2

50

50

2

10

24

6

0.453

0.453 ± 0.002

0.458

3

48

50

1.4

0

33.6

5.8

0.44

0.445 ± 0.005

0.444

4

48

50

1.4

10

33.6

5.7

0.424

0.425 ± 0.003

0.43

5

48

50

1.4

20

33.6

5.8

0.423

0.423 ± 0.006

0.423

6

49

50

1

40

33.6

6

0.418

0.419 ± 0.002

0.411

7

48

50

1.5

20

33.6

5.8

0.412

0.413 ± 0.001

0.415

  1. ANN R2 = 0.989
  2. ANN AAD = 0.059%
  3. RSM R2 = 0.95
  4. RSM AAD = 0.078%