No. | Growth temperature (°C) | Medium volume (ml) | Inoculum size (%) | Agitation rate (rpm) | Incubation period (h) | Initial pH | ANN predicted activity (Uml-1) | Actual activity ± SD (Uml-1) | RSM predicted activity (Uml-1) |
---|---|---|---|---|---|---|---|---|---|
1 | 52.3 | 50 | 1 | 0 | 24 | 5.8 | 0.47 | 0.47 ± 0.003 | 0.476 |
2 | 50 | 50 | 2 | 10 | 24 | 6 | 0.453 | 0.453 ± 0.002 | 0.458 |
3 | 48 | 50 | 1.4 | 0 | 33.6 | 5.8 | 0.44 | 0.445 ± 0.005 | 0.444 |
4 | 48 | 50 | 1.4 | 10 | 33.6 | 5.7 | 0.424 | 0.425 ± 0.003 | 0.43 |
5 | 48 | 50 | 1.4 | 20 | 33.6 | 5.8 | 0.423 | 0.423 ± 0.006 | 0.423 |
6 | 49 | 50 | 1 | 40 | 33.6 | 6 | 0.418 | 0.419 ± 0.002 | 0.411 |
7 | 48 | 50 | 1.5 | 20 | 33.6 | 5.8 | 0.412 | 0.413 ± 0.001 | 0.415 |