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Table 1 Experimental design used in RSM and ANN studies by using six independent variables showing observed values of lipase activity

From: A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM

Temperature (°C) Medium volume (ml) Inoculum size (%) Agitation rate (rpm) Incubation period (h) Initial pH Lipase activity (Uml-1)
60 162.5 4 50 60 6 0.2063
60 162.5 2 150 36 8 0
60 87.5 4 50 60 8 0.0207
50 162.5 2 150 60 8 0
60 87.5 4 150 36 8 0
60 162.5 2 50 60 8 0.0291
60 87.5 2 150 36 6 0
50 87.5 4 50 36 8 0.0886
50 162.5 2 50 36 8 0.0724
50 87.5 4 150 60 8 0
50 162.5 4 150 60 6 0.0907
50 87.5 2 50 36 6 0.1945
45 125 3 100 48 7 0.04
65 125 3 100 48 7 0.0229
55 50 3 100 48 7 0.0457
55 200 3 100 48 7 0.0551
55 125 1 100 48 7 0
55 125 5 100 48 7 0.0841
55 125 3 0 48 7 0.0675
55 125 3 200 48 7 0
55 125 3 100 24 7 0.0851
55 125 3 100 72 7 0.0281
55 125 3 100 48 5 0
55 125 3 100 48 9 0
55 125 3 100 48 7 0.049
55 125 3 100 48 7 0.0475
55 125 3 100 48 7 0.046
55 125 3 100 48 7 0.0455
55 125 3 100 48 7 0.0445
60 162.5 4 150 36 6 0
50 162.5 4 50 36 6 0.0693
60 87.5 2 50 60 6 0.0792
50 87.5 2 150 60 6 0.0996
  1. ANN training set: normal and italic (center points) numbers
  2. ANN testing set: bold numbers