Table 1 Experimental design used in RSM and ANN studies by using six independent variables showing observed values of lipase activity
Temperature (°C) | Medium volume (ml) | Inoculum size (%) | Agitation rate (rpm) | Incubation period (h) | Initial pH | Lipase activity (Uml-1) |
|---|---|---|---|---|---|---|
60 | 162.5 | 4 | 50 | 60 | 6 | 0.2063 |
60 | 162.5 | 2 | 150 | 36 | 8 | 0 |
60 | 87.5 | 4 | 50 | 60 | 8 | 0.0207 |
50 | 162.5 | 2 | 150 | 60 | 8 | 0 |
60 | 87.5 | 4 | 150 | 36 | 8 | 0 |
60 | 162.5 | 2 | 50 | 60 | 8 | 0.0291 |
60 | 87.5 | 2 | 150 | 36 | 6 | 0 |
50 | 87.5 | 4 | 50 | 36 | 8 | 0.0886 |
50 | 162.5 | 2 | 50 | 36 | 8 | 0.0724 |
50 | 87.5 | 4 | 150 | 60 | 8 | 0 |
50 | 162.5 | 4 | 150 | 60 | 6 | 0.0907 |
50 | 87.5 | 2 | 50 | 36 | 6 | 0.1945 |
45 | 125 | 3 | 100 | 48 | 7 | 0.04 |
65 | 125 | 3 | 100 | 48 | 7 | 0.0229 |
55 | 50 | 3 | 100 | 48 | 7 | 0.0457 |
55 | 200 | 3 | 100 | 48 | 7 | 0.0551 |
55 | 125 | 1 | 100 | 48 | 7 | 0 |
55 | 125 | 5 | 100 | 48 | 7 | 0.0841 |
55 | 125 | 3 | 0 | 48 | 7 | 0.0675 |
55 | 125 | 3 | 200 | 48 | 7 | 0 |
55 | 125 | 3 | 100 | 24 | 7 | 0.0851 |
55 | 125 | 3 | 100 | 72 | 7 | 0.0281 |
55 | 125 | 3 | 100 | 48 | 5 | 0 |
55 | 125 | 3 | 100 | 48 | 9 | 0 |
55 | 125 | 3 | 100 | 48 | 7 | 0.049 |
55 | 125 | 3 | 100 | 48 | 7 | 0.0475 |
55 | 125 | 3 | 100 | 48 | 7 | 0.046 |
55 | 125 | 3 | 100 | 48 | 7 | 0.0455 |
55 | 125 | 3 | 100 | 48 | 7 | 0.0445 |
60 | 162.5 | 4 | 150 | 36 | 6 | 0 |
50 | 162.5 | 4 | 50 | 36 | 6 | 0.0693 |
60 | 87.5 | 2 | 50 | 60 | 6 | 0.0792 |
50 | 87.5 | 2 | 150 | 60 | 6 | 0.0996 |