A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM

Table 1 Experimental design used in RSM and ANN studies by using six independent variables showing observed values of lipase activity

Temperature (°C)	Medium volume (ml)	Inoculum size (%)	Agitation rate (rpm)	Incubation period (h)	Initial pH	Lipase activity (Uml^-1)
60	162.5	4	50	60	6	0.2063
60	162.5	2	150	36	8	0
60	87.5	4	50	60	8	0.0207
50	162.5	2	150	60	8	0
60	87.5	4	150	36	8	0
60	162.5	2	50	60	8	0.0291
60	87.5	2	150	36	6	0
50	87.5	4	50	36	8	0.0886
50	162.5	2	50	36	8	0.0724
50	87.5	4	150	60	8	0
50	162.5	4	150	60	6	0.0907
50	87.5	2	50	36	6	0.1945
45	125	3	100	48	7	0.04
65	125	3	100	48	7	0.0229
55	50	3	100	48	7	0.0457
55	200	3	100	48	7	0.0551
55	125	1	100	48	7	0
55	125	5	100	48	7	0.0841
55	125	3	0	48	7	0.0675
55	125	3	200	48	7	0
55	125	3	100	24	7	0.0851
55	125	3	100	72	7	0.0281
55	125	3	100	48	5	0
55	125	3	100	48	9	0
55	125	3	100	48	7	0.049
55	125	3	100	48	7	0.0475
55	125	3	100	48	7	0.046
55	125	3	100	48	7	0.0455
55	125	3	100	48	7	0.0445
60	162.5	4	150	36	6	0
50	162.5	4	50	36	6	0.0693
60	87.5	2	50	60	6	0.0792
50	87.5	2	150	60	6	0.0996

ISSN: 1472-6750