Figure 1
From: Hairy-root organ cultures for the production of human acetylcholinesterase
a: Expression cassette driving the production of human AChE-R ER in transgenic plants and hairy-root cultures. L, left border; R, right border; Ag7, the nopaline synthase gene's 3' UTR; nptII, the kanamycin resistance gene; NOS, the nopaline synthase gene's promoter; 2 × 35S, cauliflower mosaic virus 35S promoter with duplicated enhancer; TEV, the translation enhancer region of the tobacco etch virus; AChE-RER, codon-optimized coding region for human AChE-R containing the C-terminal SEKDEL ER retention signal; VSP, the 3' UTR of the soybean vegetative storage protein. b: Levels of AChE-RER expression in several independent hairy-root clones.