GFP reporter expression in flies dying from dessication/starvation. Single male flies were placed in empty culture vials and tracked for 48 hours. Black/white bars indicate the light/dark cycle the flies were cultured under prior to the beginning of the experiment. Activity is expressed as distance moved per hour (blue) and GFP intensity is expressed as average pixel intensity per hour (green). Arrows indicate the last time point at which the animals displayed spontaneous movement; these animals were scored as dead at the end of the experiment based on lack of movement in response to stimulation. (A) hsp22-GFP, transgenic strain 1MI1. (B) 3xP3-GFP, transgenic strain I4. The experiment was repeated with similar results.