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Table 2 PCR primers used for plasmid construction

From: Large-scale preparation of active caspase-3 in E. coli by designing its thrombin-activatable precursors

Primer

5' → 3'

P1

GGGAATTCCATATGGAGAACACTGAAAACTCAG

P2

CCGCTCGAGGTGATAAAAATAGAGTTCTTTTGT

P3

GGGAATTCCATATGTCTGGAATATCCCTGGACAACAGT

P4

ATCAACGCTGCCGCGCGGCACCAGGCCACAGTCCAGTTCTGTACCACG

P5

TGTGGCCTGGTGCCGCGCGGCAGCGTTGATGATGACATGGCGTGTCAT

P6

ACTGTTGTCCAGGGATATGCTGCCGCGCGGCACCAGGCTTCCATGTATGATCTT

P7

GACTGTGGCATTGAGACAGCGAGTGGTGTTGATGAT

P8

CAGATCATCAACACCACTCGCTGTCTCAATGCCACA

P9

ACAGACAGTGGTGTTGATGATCTGGTGCCGCGCGGCAGCGACATGGCGTGTCATAAA

P10

ACAGACAGTGGTGTTGATGATCTGGTGCCGCGCGGCAGCGACATGGCGTGTCATAAA

  1. * NdeI restriction sites in P1 and P3, and XhoI site in P2 are underlined. Thrombin recognition sites in P4, P5, P6, P9 and P10 and Ala mutations in P7 and P8 are italicized.
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BMC Biotechnology

ISSN: 1472-6750

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