Table 1 Catalytic parameters of the caspase-3 mutants
ID | Caspase | KM(μM) | kcat(s-1) | kcat/KM (M-1s-1) |
|---|---|---|---|---|
I | Wild type | 14.16 ± 1.5 | 7.21 ± 0.93 | (5.0 ± 0.1) × 105 |
II | Δ28 | 16.38 ± 3.6 | 3.39 ± 0.58 | (2.1 ± 0.1) × 105 |
III | Δ28/175TS | 93.20 ± 33.2 | 0.05 ± 0.02 | (5.0 ± 0.0) × 102 |
IV | 28TS/175TS | 93.49 ± 9.2 | 0.02 ± 0.00 | (1.7 ± 0.0) × 102 |
V | 28TS/180TI | 18.09 ± 1.3 | 1.26 ± 0.03 | (6.7 ± 0.9) × 104 |
VI | Δ28/175TS processed with thrombin | 41.13 ± 1.9 | 1.86 ± 0.09 | (4.5 ± 0.0) × 104 |
VII | 28TS/175TS processed with thrombin | 27.81 ± 5.7 | 0.78 ± 0.06 | (2.9 ± 0.4) × 104 |
VIII | 28TS/180TI processed with thrombin | 21.39 ± 1.9 | 1.77 ± 0.13 | (8.3 ± 0.1) × 104 |