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Table 1 Catalytic parameters of the caspase-3 mutants

From: Large-scale preparation of active caspase-3 in E. coli by designing its thrombin-activatable precursors

ID Caspase KM(μM) kcat(s-1) kcat/KM (M-1s-1)
I Wild type 14.16 ± 1.5 7.21 ± 0.93 (5.0 ± 0.1) × 105
II Δ28 16.38 ± 3.6 3.39 ± 0.58 (2.1 ± 0.1) × 105
III Δ28/175TS 93.20 ± 33.2 0.05 ± 0.02 (5.0 ± 0.0) × 102
IV 28TS/175TS 93.49 ± 9.2 0.02 ± 0.00 (1.7 ± 0.0) × 102
V 28TS/180TI 18.09 ± 1.3 1.26 ± 0.03 (6.7 ± 0.9) × 104
VI Δ28/175TS processed with thrombin 41.13 ± 1.9 1.86 ± 0.09 (4.5 ± 0.0) × 104
VII 28TS/175TS processed with thrombin 27.81 ± 5.7 0.78 ± 0.06 (2.9 ± 0.4) × 104
VIII 28TS/180TI processed with thrombin 21.39 ± 1.9 1.77 ± 0.13 (8.3 ± 0.1) × 104