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Table 2 Molecular Weight and pI for the proteins examined here.

From: Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle

Characteristics

GvpC

Tat

Rev

Nef1

pI

3.73

4.92

8.17

5.31

pI (Fusion proteins)

 

3.83

3.88

4.07

MW (Da)

42,392

5,743

9,313

24,733

Expected MW †

 

48,117

51,687

67,106

Predicted MW ‡

60,000

65,743

69,313

84,733

  1. † Expected MW is calculated as GvpC + SIV insert; Tat, Rev and Nef1 respectively.
  2. ‡ Predicted MW is based on results for GvpC from wild type GV (NRC-1) where the protein band was indicated at ~ 60 kDa [18]. Accordingly, the MW for the recombinant proteins was calculated as a sum of the specific insert and the MW for the GvpC. Isoelectric points were calculated using the Web page for pI/MW calculation [42].