Characteristics | GvpC | Tat | Rev | Nef1 |
---|
pI
| 3.73 | 4.92 | 8.17 | 5.31 |
pI (Fusion proteins)
| Â | 3.83 | 3.88 | 4.07 |
MW (Da)
| 42,392 | 5,743 | 9,313 | 24,733 |
Expected MW
â€
| Â | 48,117 | 51,687 | 67,106 |
Predicted MW
‡
| 60,000 | 65,743 | 69,313 | 84,733 |
- †Expected MW is calculated as GvpC + SIV insert; Tat, Rev and Nef1 respectively.
- ‡ Predicted MW is based on results for GvpC from wild type GV (NRC-1) where the protein band was indicated at ~ 60 kDa [18]. Accordingly, the MW for the recombinant proteins was calculated as a sum of the specific insert and the MW for the GvpC. Isoelectric points were calculated using the Web page for pI/MW calculation [42].