|
Characteristics
|
GvpC
|
Tat
|
Rev
|
Nef1
|
|---|
|
pI
|
3.73
|
4.92
|
8.17
|
5.31
|
|
pI (Fusion proteins)
| |
3.83
|
3.88
|
4.07
|
|
MW (Da)
|
42,392
|
5,743
|
9,313
|
24,733
|
|
Expected MW
†
| |
48,117
|
51,687
|
67,106
|
|
Predicted MW
‡
|
60,000
|
65,743
|
69,313
|
84,733
|
- † Expected MW is calculated as GvpC + SIV insert; Tat, Rev and Nef1 respectively.
- ‡ Predicted MW is based on results for GvpC from wild type GV (NRC-1) where the protein band was indicated at ~ 60 kDa [18]. Accordingly, the MW for the recombinant proteins was calculated as a sum of the specific insert and the MW for the GvpC. Isoelectric points were calculated using the Web page for pI/MW calculation [42].
