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Table 1 Primers sequences for PCR amplification of DNA encoding the proteins examined here.

From: Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle

Fragments

Primers

Sequence (5' to 3')

Size (bp)

Tat

Tat F

ggg atg gag aca ccc ttg aag

150

 

Tat R

ggg atg ata gca aca cct ctt

 

Rev

Rev F

ggg tca gca gat ccc tat cca

243

 

Rev R

ggg cgg act ctt tgc aac gtc

 

Nef1

Nef1 F

ggg atg ggt ggc gct att tcc

642

 

Nef1 R

ggg ctt cca tgc cag tac ctc

 

gvpC

gvpC F

ctc ctg ctg tga ttc tgc ga

1,070

 

gvpC R

cat cct cac cgt act cgt cg

 
 

gvpC – Kpn I

cta tgg cca cga gat cac g

350

  1. DNA sequences required to generate recombinant GvpC and demonstrate the successful insertion and retention of test SIV DNA sequences utilized PCR amplification and the forward (F) and reverse(R) primers listed above.