Figure 6

Verification that recombinant gas vesicles have GvpC proteins containing both halobacterial and SIV encoded peptides. Western blots of putative recombinant gas vesicle samples from cultures of SD109 transformed with pMS104D::tat or ::rev demonstrate the presence of chimeric GvpC proteins. Electroblotted wild type gas vesicles samples (wt-GV) prepared from the NRC-1 were incubated with purified rabbit anti-GvpC antibody or with monkey anti-SHIV antibody. As shown, the GvpC protein is clearly detected by anti-GvpC antibody (Panel A), but this protein is not detected by anti-SHIV antibody (Panel B). In cell lysates, Tat and Rev sequences contained in the putative recombinant GvpC protein are each detected using anti-SHIV antibody (plasma number R94085) from a monkey infected in vivo with SHIV virus (Panel C). The recombinant chimeric protein bands identified here correspond in size to bands identified with anti-GvpC antibody (see Figure 5 Panel B).