Figure 6

Expression of PAP85 in Arabidopsis as represented by histochemical localization of GUS activity. Various tissues of a transgenic Arabidopsis line encoding the PAP85::GUS reporter construct pSW27 were stained for GUS activity. (A) Flower at one day post-anthesis. (B-H) Developing embryos dissected from siliques collected at 3 (B), 4 (C), 5 (D), 6 (E), 8 (F), 10 (G), and 12 (H) days post-anthesis. (I) Endosperm (e) and testa (t) from developing seed collected at 8 DPA. (J-M) Seedlings collected at 1 (J), 3 (K), 6 (L) and 10 (M) days post-germination. (N) Trichomes on primary leaf of seedling in (M). (O) Transcript abundance of PAP85 in Arabidopsis tissues. Gene expression data was extracted from the AtGenexpress Visualisation Tool (http://jsp.weigelworld.org/expviz/expviz.jsp) and Y-axis represents intensity of microarray signal in arbitrary units. Seed developmental stage approximates the time-scale of hours post-anthesis employed by Schmid et al. [42] as days post-anthesis (DPA) so as to enable more direct comparison between panels B-H and the published microarray data represented here.