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Figure 1 | BMC Biotechnology

Figure 1

From: Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems

Figure 1

Rescue of genomic coding region into a Gateway Entry vector. The Entry vector is amplified by PCR using primers that incorporate 50 bp of homology corresponding to the 5' and 3' ends of the coding region for the gene of interest. The vector amplicon is transformed into an E. coli strain possessing a BAC containing the gene of interest and expressing the bacteriophage lambda Red homologous recombination proteins. Selection for the Entry vector identifies clones containing the desired genomic coding region.

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