Demonstration of immunoreactivity with NOD mouse diabetic sera to algal-derived hGAD65. Purified algal-derived hGAD65 was coated onto 96-well plates and incubated with serum samples from diabetic NOD mice (n = 3) and reference BALB/c mice (n = 3). Bound anti-GAD antibody was detected with HRP-conjugated anti-mouse IgG. Error bars represent the standard deviation. ** above bars indicates a significant difference from reference BALB/c sera (p < 0.05).