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Figure 4 | BMC Biotechnology

Figure 4

From: Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions

Figure 4

Photostability of HyPer5 in array rescanning experiment. The performance of HyPer5 to Cy5 is compared under similar environmental condition by rescanning BAC CGH array. Ozone readings were monitored to rule out degradation from ozone effects. HyPer5/Cy3 array data is shown on the left, whereas, Cy5/Cy3 arrays are depicted on the right. Rescanning results are shown from scan 1 (top arrays), scan 4, scan 5, and scan 7 (bottom arrays). Rescanning causes drop in Cy5 signal over time resulting in the inability of Cy5 arrays to detect aberration. In contrast, HyPer5 arrays can be rescanned multiple times without any loss of aberration detection.

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