Figure 3From: Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditionsPerformance of HyPer5 dye in array CGH analysis. Genomic DNA isolated from three patients were labelled separately with Cy5-dCTP and HyPer5-CTP using BioPrime Array CGH Genomic DNA Labeling system (Invitrogen, Carlsbad, CA). The control reactions were labelled with Cy3-dCTP. The HyPer5/Cy3 arrays are shown on the left and Cy5/Cy3 arrays on the right. Results from patient 1 are on top, patient 2 in the middle and patient 3 data are shown at the bottom arrays. Samples labelled with HyPer5-dCTP perform similarly to Cy5-labeled samples in 3K BAC CGH array as demonstrated by detection of aberrations at 2p21-16.3 and 15q26.3-26.2. The y-axis represents ratios of HyPer5/Cy3 or Cy5-Cy3 signal intensities in the logarithmic scale.Back to article page