Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells

Table 1 Characterization of HCMV Donor Sera

Donor	BEIA-CMV^a	gB^b	CMV neutralization titer^c
CMV5	+	+	1:42
CMV6	+	-	1:12
CMV7	+	+	1:105
CMV8	+	-	1:20
CMV9	-	-	1:15

a. BEIA CMV ELISA detects human IgG that bind to total protein lysates from HCMV virions.
b. gB ELISA detects human IgG that bind to the AD-2 region of the HCMV envelope glycoprotein gB.
c. Sera were tested in the HCMV microneutralization assay using the AD169 strain and HELF [48]. Numbers indicate dilution of serum required for 50% inhibition of HCMV infectivity.

ISSN: 1472-6750