Sequential addition of curcuminoids to 5% BSA or FCS. A) Solid or DMSO-dissolved curcuminoids were added individually or sequentially to 5% BSA (left bracket) or FCS (right bracket) as described in Methods. After each addition the concentration of total soluble curcumin in the protein solutions was determined. B) Elution profiles of curcuminoids after single (DMSO, SOLID) or sequential (DMSO-SOLID, SOLID-DMSO) addition of DMSO-dissolved (DMSO) or solid (SOLID) curcuminoids to 5% BSA. C) Elution profiles of curcuminoids after single or sequential addition of DMSO-dissolved or solid curcuminoids to FCS. Vertical bars represent mAUs and individual curcuminoids are designated as described in Fig. 1C.