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Figure 2 | BMC Biotechnology

Figure 2

From: Recombinant amyloid beta-peptide production by coexpression with an affibody ligand

Figure 2

Purification of MAβ peptides. SDS-PAGE on a 16.5% Tris-Tricine gel at 4°C. Lane 1: Marker. 2: Cell lysate after MAβ(1–40) coexpression. 3: IMAC wash fraction (10 mM imidazole). 4: IMAC eluate after addition of 150 mM imidazole, demonstrating the effective capture of the ZAβ3:MAβ(1–40) complex. 5: Purified MAβ(1–40). 6: Purified MAβ(1–42). Samples were incubated for 2 min at 95°C prior to loading.

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