Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens

BMC Biotechnology

Table 1 Characteristics of dairy Pseudomonas spp. used

Strain	Species identification	Origin	Ribotype	Prot/Le/Li	Source or reference
28	P. fluorescens	RSMT	*	+//	PT unpublished
33	P. fluorescens	RSMT	*	+//	PT unpublished
33B	P. fluorescens	RSMT	*	+//	PT unpublished
35	P. fluorescens	RSMT	*	+//	PT unpublished
37	P. fluorescens	RSMT	*	+//	PT unpublished
37B	P. fluorescens	RSMT	*	+//	PT unpublished
7	P. fluorescens	Teat cup shell	*	//*	PT unpublished
8	P. fluorescens	Raw milk	*	//*	PT unpublished
D3-149^‡	P. fluorescens	Raw milk	409-S-3	+/+/+	[3]
D3-197^‡	P. fluorescens	Processed milk	422-S-2	+/-/-	[3]
D3-331^‡	P. fluorescens	Floor	57-S-8	+/+/+	[3]
D3-175^‡	P. fluorescens	Processed milk	408-S-8	+/+/+	[3]
D1-045^‡	P. fluorescens	Processed milk	50-S-8	+/+/+	[10]
B1-020^‡	P. fluorescens	Potato isolate	57-S-5	-/-/+	[10]
D1-027^‡	P. fulva	Raw milk	53-S-5	-/-/-	[10]
D2-160^‡	P. putida	Raw milk	112-S-7	-/-/-	[10]
B1-041^‡	P. putida	Raw milk	50-S-7	-/-/-	[10]
D1-026^‡	P. putida	Raw milk	94-S-6	-/-/-	[10]
D1-046^‡	P. fragi	Raw milk	72-S-3	-/-/-	[10]
B1-020^‡	P. fragi	Raw milk	50-S-6	-/-/-	[10]

Isolate characteristics include origin, ribotypes and extracellular enzyme production [Protease (Prot), Lecithinase (Le) and Lipase (Li)]
RSMT – rubber short milk tube that connects the teatcup assemblies to the claw. * not performed, + positive for enzyme activity, - negative for enzyme activity.
^‡ kindly provided by K. Boor (Milk Quality Improvement Program, Department of Food Science, Cornell University, Ithaka, N.Y.). All other bacterial strains were isolated from the dairy industry plant in Pacos de Ferreira, Portugal.

ISSN: 1472-6750