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Figure 4 | BMC Biotechnology

Figure 4

From: One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

Figure 4

On-column refolding and thioester generation. Schematic representation of the simultaneous on-column refolding and thioester generation method. Following capture on a chitin resin incubation of RNase A – intein – CBD with MESNA and diMESNA results in simultaneous refolding of the RNase A and cleavage of the RNase A from the intein domain, generating a C-terminal MESNA thioester. The order of refolding and thioester generation is arbitrary.

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BMC Biotechnology

ISSN: 1472-6750

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