Figure 1
GFP thioester exchange. a) Deconvoluted mass spectrum obtained from ESI-MS analysis of GFP-MESNA incubated in glutathione buffer (3 mM GSH, 1 mM GSSG) for 10 h at RT. The peaks at 27870 Da and 27579 Da correspond to the glutathione-thioester of GFP (MWcalc = 27869 Da) and hydrolyzed GFP with a carboxyl C-terminus (MWcalc = 27576 Da), respectively. b) Deconvoluted mass spectrum obtained from ESI-MS analysis of GFP-MESNA incubated in MESNA refolding buffer (3 mM MESNA, 1 mM diMESNA) for 10 h at RT. The peak 27706 Da corresponds to the MESNA-thioester of GFP (MWcalc = 27704 Da).