Figure 2

a – Assays of ApoE production. Some conditions known to induce ApoE production in other cells and their effect upon MSCs. NSC conditioned media AIM and osteogenic conditions are also included. Experiment was repeated with 2 donors and data from one of the donors is presented, n = 3, average ± standard deviation is shown. b – Detection of ApoE in MSCs by western blotting. Western blot demonstrating ApoE (34 kD) production (upper) in early (passage 2, left column) and late (passage 8, right column) passage MSCs. Lanes were normalized to GAPDH expression (lower). Experiment was repeated 5 donors. c – Dex-mediated expression of ApoE in MSCs is not accompanied by adipocyte differentiation. ApoE expression by MSCs was induced by exposure to Dex or AIM. After 21 days, the monolayers were stained with oil red O to visualize lipid droplets (left). Lipid droplets were evident in AIM treated, but not Dex treated MSCs. However, ApoE expression was high in both cases when media was assayed by ELISA (right).