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Figure 2 | BMC Biotechnology

Figure 2

From: Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin

Figure 2

Immunocytochemistry assay of EGFR expression and Western-blot analysis of the specific FAK gene silencing by EGF-targeted phagemid particles mediated RNA interference. A: In the immunocytochemical assay, H1299 cells showed a strong positive EGFR immunoreactivity, while very light immunostaining was observed in the U87 cells that were used as negative controls. 1, H1299; 2, U87. B: H1299 cells were infected with pSi4.1-siFAK phagemid particles. In HCPT treated-cells, the pSi4.1-siFAK phagemid particles could inhibit FAK expression to a great extent. 1, H1299; 2, H1299 infected with pSi4.1-simock phagemid particles; 3, H1299 infected with pSi4.1-simock phagemid particles in the presence of 2.5 μM HCPT; 4, H1299 infected with pSi4.1-siFAK phagemid particles; 5, H1299 infected with pSi4.1-siFAK phagemid particles in the presence of 2.5 μM HCPT; 6, H1299 transfected with pSi4.1-siFAK using Lipofectamine 2000.

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BMC Biotechnology

ISSN: 1472-6750

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