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Table 4 Specific activities, production and iron quantitation assay of purified CrHydA1 anaerobically expressed in S. oneidensis AS52A1N strain grown with DMSO, TMAO or thiosulfate as substrates for anaerobic respiration.

From: Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Anaerobic respiration substrate used for cell growth Activity of HydA1 μmol H2 mg-1 min-1 Atoms of Fe/molecule of protein Production of HydA1 protein μg/L of culture(OD600 = 1)
TMAO 10 mM 705 ± 18 6.4 ± 0.5 400 ± 20
Thiosulfate 20 mM 695 ± 33 5.89 ± 0.32 420 ± 35
DMSO 10 mM 740 ± 56 6.08 ± 0.12 500 ± 28
  1. Values are: result ± standard deviation (at least three replicates of all experiments).