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Table 4 Specific activities, production and iron quantitation assay of purified CrHydA1 anaerobically expressed in S. oneidensis AS52A1N strain grown with DMSO, TMAO or thiosulfate as substrates for anaerobic respiration.

From: Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Anaerobic respiration substrate used for cell growth

Activity of HydA1 μmol H2 mg-1 min-1

Atoms of Fe/molecule of protein

Production of HydA1 protein μg/L of culture(OD600 = 1)

TMAO 10 mM

705 ± 18

6.4 ± 0.5

400 ± 20

Thiosulfate 20 mM

695 ± 33

5.89 ± 0.32

420 ± 35

DMSO 10 mM

740 ± 56

6.08 ± 0.12

500 ± 28

  1. Values are: result ± standard deviation (at least three replicates of all experiments).