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Table 3 Hydrogen formation from S. oneidensis cells grown in the presence of various substrates for anaerobic respiration.

From: Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Anaerobic respiration substrate used for cell growth Strain AS52A1N μmol H2 min-1 ml culture-1 (OD600 = 1) Strain AS51 μmol H2 min-1 ml culture-1 (OD600 = 1) Strain AS50 μmol H2 min-1 ml culture-1 (OD600 = 1)
Ferric citrate 10 mM 0.05 ± 0.0045 0.031 ± 0.0032 0.034 ± 0.0025
Nitrate 10 mM 0.035 ± 0.002 0.029 ± 0.003 0.04 ± 0.003
Fumarate 20 mM 0.061 ± 0.004 0.06 ± 0.0056 0.042 ± 0.004
TMAO 10 mM 1.4 ± 0.13 0.62 ± 0.05 0.022 ± 0.001
Thiosulfate 20 mM 1.6 ± 0.28 0.8 ± 0.09 0.019 ± 0.001
DMSO 10 mM 0.83 ± 0.046 0.34 ± 0.015 0.02 ± 0.001
No addition 0.02 ± 0.001 0.023 ± 0.002 0.023 ± 0.002
  1. Values are: result ± standard deviation (at least three replicate cultures for all conditions).