Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Table 3 Hydrogen formation from S. oneidensis cells grown in the presence of various substrates for anaerobic respiration.

Anaerobic respiration substrate used for cell growth	Strain AS52A1N μmol H₂ min^-1 ml culture^-1 (OD₆₀₀ = 1)	Strain AS51 μmol H₂ min^-1 ml culture^-1 (OD₆₀₀ = 1)	Strain AS50 μmol H₂ min^-1 ml culture^-1 (OD₆₀₀ = 1)
Ferric citrate 10 mM	0.05 ± 0.0045	0.031 ± 0.0032	0.034 ± 0.0025
Nitrate 10 mM	0.035 ± 0.002	0.029 ± 0.003	0.04 ± 0.003
Fumarate 20 mM	0.061 ± 0.004	0.06 ± 0.0056	0.042 ± 0.004
TMAO 10 mM	1.4 ± 0.13	0.62 ± 0.05	0.022 ± 0.001
Thiosulfate 20 mM	1.6 ± 0.28	0.8 ± 0.09	0.019 ± 0.001
DMSO 10 mM	0.83 ± 0.046	0.34 ± 0.015	0.02 ± 0.001
No addition	0.02 ± 0.001	0.023 ± 0.002	0.023 ± 0.002

Values are: result ± standard deviation (at least three replicate cultures for all conditions).

ISSN: 1472-6750