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Table 2 hydA1 rare codons occurrences in organisms with sequenced genomes which have putative homologues of C. reinhardtii hydA1, hydEF and hydG.

From: Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Organisms Rare codons occurrence less than 2/1000 Rare codons occurrence less than 5/1000
Shewanella oneidensis 0 4
Escherichia coli a 2 4
Bacteroides thetaiodaomicron, 0 6
Desulfovibrio desulfuricans 4 7
Desulfovibrio vulgaris 2 19
Chlamydomonas reinhardtii 2 21
Thermotoga maritima 26 40
Clostridium thermocellum 4 42
Thermoanaerobacter tengcongensis 4 78
Trichomonas vaginalis 108 162
Clostridium acetobutylicum 43 209
C. pasteurianum 62 209
C. difficile 76 279
C. tetani 121 303
C. perfringens 143 313
C. botulinum 168 316
  1. aE. coli has no putative homologues of C. reinhardtii hydA1, hydEF and hydG, but was previously used for HydA1 heterologous overexpression. Gene number was found within the following reference: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, organism name was found within reference: http://www.kazusa.or.jp/codon/. The script is available as open-source software at: http://sourceforge.net/projects/codons.