Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Table 2 hydA1 rare codons occurrences in organisms with sequenced genomes which have putative homologues of C. reinhardtii hydA1, hydEF and hydG.

Organisms	Rare codons occurrence less than 2/1000	Rare codons occurrence less than 5/1000
Shewanella oneidensis	0	4
Escherichia coli ^a	2	4
Bacteroides thetaiodaomicron,	0	6
Desulfovibrio desulfuricans	4	7
Desulfovibrio vulgaris	2	19
Chlamydomonas reinhardtii	2	21
Thermotoga maritima	26	40
Clostridium thermocellum	4	42
Thermoanaerobacter tengcongensis	4	78
Trichomonas vaginalis	108	162
Clostridium acetobutylicum	43	209
C. pasteurianum	62	209
C. difficile	76	279
C. tetani	121	303
C. perfringens	143	313
C. botulinum	168	316

^aE. coli has no putative homologues of C. reinhardtii hydA1, hydEF and hydG, but was previously used for HydA1 heterologous overexpression. Gene number was found within the following reference: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, organism name was found within reference: http://www.kazusa.or.jp/codon/. The script is available as open-source software at: http://sourceforge.net/projects/codons.

ISSN: 1472-6750