Table 1 Strains and plasmids used in this study.

E. coli

   XL1-Blue

recA, endA1, gyrA96, thi, hsdR17 (rk-, mk+), supE44, relA1, lambda-, lac-, [F', proAB, lacIqZdeltaM15, Tn10(tet)]

13

S. oneidensis

   MR1

Wild type

31

   AS50

In-frame deletion of hydA(SO3920) in MR1

21

   AS51

In-frame deletion of hyaB (SO2098) in AS92 [31]; Gm^r

21

   AS52

In-frame deletion of hydA (SO3920) and hyaB (SO2098) in MR1

21

   AS521AN

AS52 transformed with pBBR-hydA1N; Kan^r

This study

   AS521AC

AS52 transformed with pBBR-hydA1C; Kan^r

This study

Plasmids

   pBBR1-MCS2

Broad-host-range plasmid, LacI^-, multiple cloning site in LacZ; Kan^r

14

   pBBR-hydA1N

pBBR1-MCS2 with N-terminal Strep-tagII-hydA1; Kan^r

This study

   pBBR-hydA1C

pBBR1-MCS2 with C-terminal Strep-tagII-hydA1; Kan^r

This study