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Table 1 Strains and plasmids used in this study.

From: Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii

Strain or plasmid Genotype or description Source or reference
E. coli   
   XL1-Blue recA, endA1, gyrA96, thi, hsdR17 (rk-, mk+), supE44, relA1, lambda-, lac-, [F', proAB, lacIqZdeltaM15, Tn10(tet)] 13
S. oneidensis   
   MR1 Wild type 31
   AS50 In-frame deletion of hydA(SO3920) in MR1 21
   AS51 In-frame deletion of hyaB (SO2098) in AS92 [31]; Gmr 21
   AS52 In-frame deletion of hydA (SO3920) and hyaB (SO2098) in MR1 21
   AS521AN AS52 transformed with pBBR-hydA1N; Kanr This study
   AS521AC AS52 transformed with pBBR-hydA1C; Kanr This study
Plasmids   
   pBBR1-MCS2 Broad-host-range plasmid, LacI-, multiple cloning site in LacZ; Kanr 14
   pBBR-hydA1N pBBR1-MCS2 with N-terminal Strep-tagII-hydA1; Kanr This study
   pBBR-hydA1C pBBR1-MCS2 with C-terminal Strep-tagII-hydA1; Kanr This study