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Figure 5 | BMC Biotechnology

Figure 5

From: The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis

Figure 5

Stability of Tv DAO under conditions of substrate conversion. The operational stability under bubble aeration conditions is compared for soluble enzyme in absence (1; and inset) and presence of 1% Pluronic F-68 (2) and TvDAO immobilized on Strep-Tactin MacroPrep particles (3). The initial concentration of D-methionine was 20 mM and it was added in solid form to the reactor in regular intervals to prevent its depletion. A total volume of 20 mL was used; all other conditions were as described in Figure 4. The black lines for series 2 and 3 show least squares fits of a double exponential decay function to the actual stability curves which are shown in grey.

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