Figure 4
From: The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis
Interfacial inactivation of free and immobilized Tv DAO preparations. Loss of activity induced by bubble aeration in a miniaturized stirred reactor is shown for the different enzyme preparations and compared to a non-aerated control of soluble TvDAO (full down triangles). Experiments were performed at 30°C with stirring at 300 rpm and an aeration rate of 15 L/h; protein concentration was 1 μM in Tris-buffer. Symbols: full triangles, soluble enzyme; open triangles, soluble enzyme in presence of 1% Pluronic F-68; open squares, TvDAO immobilized on Strep-Tactin MacroPrep beads; full circles, TvDAO on Sepabeads EC-EP.