Figure 3
From: The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis
Release of FAD cofactor during thermally induced inactivation of different preparations of Tv DAO. The concentration of free FAD was measured in samples taken during the course of the inactivation experiment at 50°C. After removal of the enzymes by sedimentation (immobilized preparations) or ultrafiltration (soluble TvDAO) the content of FAD in solution was determined fluorometrically. Symbols are used as in Figure 2; exponential rise functions were fitted to the experimental data (solid lines). The inset compares relative inactivation (open symbols) and relative FAD release (full symbols) for TvDAO immobilized on Sepabeads EC-EP.