Table 1 Timetable of progressive deprivation of serum over the 6-day culture period of chondrocyte/agarose constructs.
From: Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression
FBS | ITS | Ascorbic acid | Hepes buffer | |
|---|---|---|---|---|
Day 0 | 10% | - | - | 10 mM |
Day 1 | 10% | - | - | 10 mM |
Day 2 | 5% | - | 5 ng/mL | 20 mM |
Day 3 | 5% | 1% | 10 ng/mL | 20 mM |
Day 4 | 1% | 1% | 15 ng/mL | 30 mM |
Day 5 | - | 1% | 20 ng/mL | 30 mM |
Day 6 | - | 1% | 20 ng/mL | 30 mM |