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Figure 7 | BMC Biotechnology

Figure 7

From: Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development

Figure 7

(a, b, c, d) Depicts cavitation bubbles (Cb) and residual surface scarring (Ss) after the applied fs laser pulses. (a) Cavitation bubble (Cb) was produced using an average laser power of 45 mW with a beam dwell time of 100 ms and a single pulsing event of the galvo. The diameter of the cavitation bubble was measured to be ~5 μm. (b) After pulsing the galvo a total of 3 times, surface scarring (Ss) of the blastomere was observed. The spatial extent of tissue scarring was measured to be ~5 μm. (c) Depicts the cavitation bubble (Cb) created using an average laser power of 45 mW with a beam dwell time of 500 ms and a single pulsing event of the galvo. Diameter of the cavitation bubble was measured to be ~10 μm. (d) After pulsing the galvo a total of 2 times, surface scarring (Ss) of the blastomere was observed. The spatial extent of tissue scarring was measured to be ~10 μm. (a, b, c, d) Still images were extracted from additional file 1. Scale bars for (a, b, c, d) represent 5 μm.

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BMC Biotechnology

ISSN: 1472-6750

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