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Figure 4 | BMC Biotechnology

Figure 4

From: Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development

Figure 4

Fluorescence and brightfield images of post-laser manipulated 8-cell stage embryos. Embryos were porated for exogenous FITC delivery using an average laser power of 45 mW. The beam dwell time was set to 50 ms with three pulsing events of the galvo for (a, b) and two for (c, d). Three pores were made in each of 2 blastomere cells, yielding a total of six pores for the entire embryo. Scale bars for (a, b, c, d) represent 200 μm.

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BMC Biotechnology

ISSN: 1472-6750

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