Magnified top and lateral SEM images of key developmental features in larvae reared to 7 dpf. (a, b, c) Depict the olfactory pit, ear, posterior forebrain and dorsal midbrain in laser-manipulated larvae. (d, e, f) Depict the olfactory pit, ear, posterior forebrain and dorsal midbrain in control larvae. (g, h) Illustrated comparison of dorso-lateral views of the posterior forebrain and dorsal midbrain in (g) a laser-manipulated and (h) a control larva at 7 dpf. Location of the eye (E), diencephalon (D) and optic tectum (OT) are shown respectively. Scale bars for (a, d) represent 20 μm and for (b, c, e, f, g, h) 50 μm. Orientation: anterior (left); posterior (right).