Key developmental features in a laser-manipulated and a control larva reared to 7 dpf. (a) SEM whole body image of a laser-manipulated larva at 7 dpf. Developmental features indicated are the ventral fin (VF), notochord (NC), pectoral fin (PF), otic capsule (OC), otic vesicle (OV), eye (E; cornea), olfactory pit (OP) and the protruding mouth (PM). (b) Magnified antero-dorsal view of the protruding mouth (PM), olfactory pit (OP), eye (E; cornea), otic capsule (OC) and otic vesicle (OV) of the larva in (a). (c) SEM whole body image of a control larva at 7 dpf. Similar developmental features observed in (a) were also seen in (c). (d) Magnified antero-lateral view of the same larva in (c). Developmental features illustrated in (b) are also shown in (d). Scale bars for (a, c) represent 200 μm and for (b, d) 100 μm.