Figure 5
Comparison of qPCR and microarray measurements for miR-296 and miR-494 titrations. Scatter plots are shown for titration of synthetic miR-296 into liver (top left panel) and placenta (top right panel) total RNAs, and miR-494 into liver (lower left) and placenta (lower right) total RNAs. Ct values from qPCR are plotted on the x-axis, while log2 of the total gene signal from microarray measurements are plotted on the y-axis. Numbers in red show the number of attomoles of spike-in miRNA per 100 ng total RNA. Equations and R values are for the orthogonal line fit of the linear regions of each titration. Error bars indicate standard deviation (SD) of Ct values for qPCR results and (SD/Mean)*log2e for the array results.