Figure 1
Comparison of qPCR and microarray miRNA profiling for individual miRNAs in nine human tissues. Scatter plots are shown for 9 of the 61 miRNAs assayed, with qPCR results (cycle threshold (Ct) values) on the x axes and microarray results (log2 of the total gene signal) on the y axes. Each data point represents one tissue. All plots are drawn to the same scale. The equations and R values on each plot are for the orthogonally-fitted line. Spot colors indicate the tissue: red = breast, pink = testes, dark blue = heart, light blue = placenta, dark green = liver, light green = ovary, orange = brain, brown = skeletal muscle, and grey = thymus. Tissues where qPCR results were flagged as "undetermined" by ABI software, or where log2 of the total gene signal on arrays was < 1, were not plotted. Error bars indicate standard deviation (SD) of Ct values for qPCR results and (SD/Mean)*log2e of the signals for the array results. Scatter plots for the remaining 51 miRNAs (one miRNA gave no signals with either qPCR or arrays) are in Additional Files 2, 3, 4, 5, 6, 7.