In in vivo assay of yCD protein. In (A), LoVo cells were seeded in 96-well plate (2500 cells/well) and cultured in BM for 4 days containing 2.5 μg ml-1 of yCD in presence of the indicated concentrations of 5-FC. In (B), the cells were culture at same conditions but in BM containing 2.5 μg ml-1 of yCD and 10 μg ml-1 of 5-FC in presence of different concentrations of scFvH5 or the irrelevant scFvGO antibodies. Cell cytotoxicity (due to de novo formed 5-FU) was evaluated by WST-1 assay and calculated as a percentage of survived cells. Values are reported as the mean of triplicate samples. The bars indicate SD.