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Figure 3 | BMC Biotechnology

Figure 3

From: Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii

Figure 3

Gene disruption of AgPRS2,4 and AgPRS3. A) Left, schematic representation of the strategy followed to achieve the disruption of the AgPRS2,4 gene (AGR371C). Right, Southern blot analysis to confirm correct PRS2,4 disruption. Genomic DNA was digested with HindIII and a genomic KpnI-HindIII fragment was used as a radioactive probe: lane 1, wild-type strain; lane 2, heterokaryotic disruptant; lane 3, mutant Δprs2,4. B) Left, schematic representation of the strategy for AgPRS3 (AGL080C) gene disruption. Right, Southern blot analysis to verify correct PRS3 disruption. Genomic DNA was digested with SacI and a genomic EcoRI fragment was used as a radioactive probe: lane 1, wild-type strain; lane 2, mutant Δprs3. HIII, HindIII; N, NcoI; HII, HincII; EV, EcoRV; K, KpnI; EI, EcoRI; S, SacI.

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