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Figure 3 | BMC Biotechnology

Figure 3

From: Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)

Figure 3

ELISA on directly immobilized inactive VEEV particles. Antigen: 1 μg VRS purified VEEV particles. A. A dilutions series of scFv phage particles were used for VEEV detection. The scFv phage were detected using mAb anti-M13 conjugated with HRP (1:5000). The mean values of two ELISAs from two independent scFv phage productions are shown. B. A series of scFv-Fc fusion protein dilutions were used for VEEV detection. The scFv-Fc were detected using goat anti-human IgG Fc specific antibody conjugated with HRP (1:20000). IIB6 is a non VEEV-specific control scFv antibody.

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BMC Biotechnology

ISSN: 1472-6750

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