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Figure 1 | BMC Biotechnology

Figure 1

From: Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)

Figure 1

ELISA with 1010 (cfu) polyclonal scFv phage of each panning round. Antigen (directly immobilized): 1 μg VRS purified VEEV particles (VEEV VRS), 1 μg VRS concentrated supernatant from non-infected Vero cells (Vero VRS), 1:2 diluted supernatant of non infected VERO cells (Vero SN), 0.5 μg of each anti-VEEV capture mAb 8747 and VEE-WIS1, 1 μg lysozyme. The bound scFv phage were detected using mAb anti-M13 conjugated with HRP (1:5000).

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