Figure 1From: Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)ELISA with 1010 (cfu) polyclonal scFv phage of each panning round. Antigen (directly immobilized): 1 μg VRS purified VEEV particles (VEEV VRS), 1 μg VRS concentrated supernatant from non-infected Vero cells (Vero VRS), 1:2 diluted supernatant of non infected VERO cells (Vero SN), 0.5 μg of each anti-VEEV capture mAb 8747 and VEE-WIS1, 1 μg lysozyme. The bound scFv phage were detected using mAb anti-M13 conjugated with HRP (1:5000).Back to article page