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Table 3 Assessment of biological activity of HEK293-produced IFNα2b using two antiviral assays

From: Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

Antiviral assay

IFNα2b activity (U/mg)

 

HEK293 (1 feed)

E. coli

HEK293 (2 feeds)

E. coli

MDBK/VSV

4.1 × 108

3.1 × 108

12.2 × 108

4.0 × 108

A549/EMCV

6.0 × 108

3.9 × 108

7.2 × 108

8.1 × 108

  1. IFNα2b activity has been determined by inhibition of viral replication of vesicular stomatitis virus (VSV) in Madin Darby bovine kidney cells (MDBK) and of encephalomyocarditis virus (ECMV) in the lung carcinoma cell line A-549. Both antiviral assays were carried out by PBL InterferonSource. In each assay performed independently, E. coli IFNα2b was used as a positive control.
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BMC Biotechnology

ISSN: 1472-6750

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